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marathon ready human brain cdna library  (TaKaRa)


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    TaKaRa marathon ready human brain cdna library
    Molecular cloning of <t>novel</t> <t>RGS6</t> splice forms from human brain. ( A ) Splicing diagram of known RGS6 splice forms. Location of primers used for PCR-based amplification of RGS6 transcripts from a human whole brain <t>cDNA</t> library are shown in red. ( B ) Representative plasmid restriction digest (right) or nested PCR (down) indicating positive hits whose size is larger than known RGS6 splice forms. Each gel has a confirmed RGS6Lα1(+GGL) transcript (largest known splice form) indicated as well as yellow asterisks denoting transcripts encoding RGS6LA3α1(+GGL) which encodes RGS6B.
    Marathon Ready Human Brain Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+brain+cdna+library/Human+Pancreas+Marathon+-Ready+cDNA/bio_rxiv__64898__2026__05__08__723811-52-11-17
    Average 94 stars, based on 47 article reviews
    marathon ready human brain cdna library - by Bioz Stars, 2026-09
    94/100 stars

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    1) Product Images from "Molecular cloning of a novel, nervous system-specific RGS6 isoform lacking canonical G protein regulatory effects and with dominant negative actions"

    Article Title: Molecular cloning of a novel, nervous system-specific RGS6 isoform lacking canonical G protein regulatory effects and with dominant negative actions

    Journal: bioRxiv

    doi: 10.64898/2026.05.08.723811

    Molecular cloning of novel RGS6 splice forms from human brain. ( A ) Splicing diagram of known RGS6 splice forms. Location of primers used for PCR-based amplification of RGS6 transcripts from a human whole brain cDNA library are shown in red. ( B ) Representative plasmid restriction digest (right) or nested PCR (down) indicating positive hits whose size is larger than known RGS6 splice forms. Each gel has a confirmed RGS6Lα1(+GGL) transcript (largest known splice form) indicated as well as yellow asterisks denoting transcripts encoding RGS6LA3α1(+GGL) which encodes RGS6B.
    Figure Legend Snippet: Molecular cloning of novel RGS6 splice forms from human brain. ( A ) Splicing diagram of known RGS6 splice forms. Location of primers used for PCR-based amplification of RGS6 transcripts from a human whole brain cDNA library are shown in red. ( B ) Representative plasmid restriction digest (right) or nested PCR (down) indicating positive hits whose size is larger than known RGS6 splice forms. Each gel has a confirmed RGS6Lα1(+GGL) transcript (largest known splice form) indicated as well as yellow asterisks denoting transcripts encoding RGS6LA3α1(+GGL) which encodes RGS6B.

    Techniques Used: Molecular Cloning, Amplification, cDNA Library Assay, Plasmid Preparation, Nested PCR

    RGS6B is an RGS6L(+GGL) isoform containing A3 and the α terminal exon (RGS6LA3α1(+GGL)). ( A ) Schematic diagram of the exon splicing scheme comparing RGS6Lα1(+GGL) (RGS6L) and RGS6LA3α1(+GGL) (RGS6B). mRNA sequence conservation of exon A3 between mouse and human is depicted below with the consensus sequence from 100 vertebrate species extracted by PhyloP. PCR amplification of exon A3 containing mRNA transcripts from select mouse tissues ( B ) or human cDNA libraries ( C ). Plasmids encoding RGS6Lα1, RGS6Lα2 and RGS6LA3α1 (all +GGL) are used as negative and positive controls, respectively. LNG, lung; KDN, kidney; LVR, liver; SPL, spleen; CTX, cortex; MB, midbrain; CRB, cerebellum; HIP, hippocampus; MSC, muscle; HRT, heart; PNC, peripheral nervous system; BR, whole brain. ( D ) HEK293T cells were transfected with a plasmid encoding RGS6LA3α1(+GGL) to confirm co-migration with mouse RGS6B via immunoblotting. ( E ) shRNA or miRNA constructs targeting the A3 exon were introduced into mouse primary cortical astrocytes. The ratio of RGS6B: RGS6L was determined via immunoblot and quantified from 4 independent experiments. α Tubulin serves as a loading control for immunoblots. Data were analyzed by one sample t-test to detect deviation of each group from 100% (scramble RNAi control). *P<0.05, ***P<0.001. Data are expressed as mean ± S.E.M.
    Figure Legend Snippet: RGS6B is an RGS6L(+GGL) isoform containing A3 and the α terminal exon (RGS6LA3α1(+GGL)). ( A ) Schematic diagram of the exon splicing scheme comparing RGS6Lα1(+GGL) (RGS6L) and RGS6LA3α1(+GGL) (RGS6B). mRNA sequence conservation of exon A3 between mouse and human is depicted below with the consensus sequence from 100 vertebrate species extracted by PhyloP. PCR amplification of exon A3 containing mRNA transcripts from select mouse tissues ( B ) or human cDNA libraries ( C ). Plasmids encoding RGS6Lα1, RGS6Lα2 and RGS6LA3α1 (all +GGL) are used as negative and positive controls, respectively. LNG, lung; KDN, kidney; LVR, liver; SPL, spleen; CTX, cortex; MB, midbrain; CRB, cerebellum; HIP, hippocampus; MSC, muscle; HRT, heart; PNC, peripheral nervous system; BR, whole brain. ( D ) HEK293T cells were transfected with a plasmid encoding RGS6LA3α1(+GGL) to confirm co-migration with mouse RGS6B via immunoblotting. ( E ) shRNA or miRNA constructs targeting the A3 exon were introduced into mouse primary cortical astrocytes. The ratio of RGS6B: RGS6L was determined via immunoblot and quantified from 4 independent experiments. α Tubulin serves as a loading control for immunoblots. Data were analyzed by one sample t-test to detect deviation of each group from 100% (scramble RNAi control). *P<0.05, ***P<0.001. Data are expressed as mean ± S.E.M.

    Techniques Used: Sequencing, Amplification, Transfection, Plasmid Preparation, Migration, Western Blot, shRNA, Construct, Control

    Related Articles

    other:

    Article Title: Enzymatic basis of branching and extension of O -Man glycans for keratan sulfate biosynthesis
    Article Snippet: Point mutations (D299 F, DVF-FKI, R304 T, and H369 V for GnT-IX, and F283D, FKI-DVF, T288 R, and V341H for GnT-V) were introduced by PCR using pcDNA-IHT/human GnT-IX and pcDNA-IH/human GnT-V (T121 to C-term) ( ) as templates using the QuikChange Lightning Site-Directed Mutagenesis Kit. cDNA for full-length human B4GALT1 was amplified by PCR using reverse-transcribed total RNA from HEK293 cells as a template and cloned into pCR-BluntII-TOPO by using Zero Blunt TOPO PCR Cloning Kit (Invitrogen). cDNAs for full-length human B4GALT4, B3GNT7, CHST1, CHST2, and CHST6 were amplified by PCR using reverse-transcribed total RNA from HMCB cells (for B4GALT4, B3GNT7, and CHST6) or LN-229 cells (for CHST2), or a human brain cDNA library (TAKARA, human MTC panel 1) (for CHST1) and cloned into the EcoRI site of pcDNA6-mycHisA by Gibson assembly to construct pcDNA6-mycHisA/B4GALT4, B3GNT7, CHST1, CHST2 and CHST6.

    Article Title: Enzymatic basis of branching and extension of O-Man glycans for keratan sulfate biosynthesis.
    Article Snippet: Point mutations (D299F, DVF-FKI, R304T, and H369V for GnT-IX, and F283D, FKI-DVF, T288R, and V341H for GnT-V) were introduced by PCR using pcDNA-IHT/human GnT-IX and pcDNA-IH/human GnT-V (T121 to C-term) (42) as templates using the QuikChange Lightning SiteDirected Mutagenesis Kit. cDNA for full-length human B4GALT1 was amplified by PCR using reverse-transcribed total RNA from HEK293 cells as a template and cloned into pCR-BluntII-TOPO by using Zero BluntTM TOPOTM PCR Cloning Kit (Invitrogen). cDNAs for full-length human B4GALT4, B3GNT7, CHST1, CHST2, and CHST6 were amplified by PCR using reverse-transcribed total RNA from HMCB cells (for B4GALT4, B3GNT7, CHST6) or LN-229 cells (for CHST2), or a human brain cDNA library (TAKARA, human MTC panel 1) Jo urn al Pr e-p roo f 11 (for CHST1) and cloned into the EcoRI site of pcDNA6-mycHisA by Gibson assembly to construct pcDNA6-mycHisA/B4GALT4, B3GNT7, CHST1, CHST2 and CHST6.

    Amplification:

    Article Title: Variants in MTNAP1 underlie a neurodegenerative disorder by impairing mitochondrial stability
    Article Snippet: .. Full-length human MTNAP1 ( NM_017941.6 ) cDNA was amplified from a human brain cDNA library (Takara Bio, 637242) using gene-specific primers (Table ) incorporating restriction enzyme sites compatible with mammalian and bacterial expression vectors. .. The amplified PCR product was cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen, V79020) and the bacterial expression vector pET21b(+) (Novagen, 69741).

    Article Title: Identification of Splicing Regulatory Activity of ATXN1 and Its Associated Domains
    Article Snippet: .. In the first step, ATXN1 was amplified from the Clontech Human Brain cDNA Library (Clontech, Mountain View, CA, USA) using the primer set nest-ATXN1-Fw and nest-ATXN1-Rv. ..

    Article Title: Variants in MTNAP1 underlie a neurodegenerative disorder by impairing mitochondrial stability.
    Article Snippet: .. AR TI CL E IN P RE SS Oligonucleotide Sequence Forward primer 1 (P1 and P2) 5’-ATGAGTGATAATCCACCCAG-3’ Reverse primer 1 (P1 and P2) 5’-GCTGGCTTGGACTGATAAAC-3’ Forward primer 2 (P3) 5’-GGGAGCTGTACAAAAAGG-3’ Reverse primer 2 (P3) 5’-GAAACTCCAGCTACAACA-3’ Gene cloning and mutagenesis of MTNAP1 Full-length human MTNAP1 (NM_017941.6) cDNA was amplified from a human brain cDNA library (Takara Bio, 637242) using gene-specific primers (Table 2) incorporating restriction enzyme sites compatible with mammalian and bacterial expression vectors. .. The amplified PCR product was cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen, V79020) and the bacterial expression vector pET21b(+) (Novagen, 69741).

    Article Title: Identification of Splicing Regulatory Activity of ATXN1 and Its Associated Domains.
    Article Snippet: .. In the first step, ATXN1 was amplified from the Clontech Human Brain cDNA Library (Clontech, Mountain View, CA, USA) using the primer set nest-ATXN1-Fw and nest-ATXN1-Rv. ..

    cDNA Library Assay:

    Article Title: Variants in MTNAP1 underlie a neurodegenerative disorder by impairing mitochondrial stability
    Article Snippet: .. Full-length human MTNAP1 ( NM_017941.6 ) cDNA was amplified from a human brain cDNA library (Takara Bio, 637242) using gene-specific primers (Table ) incorporating restriction enzyme sites compatible with mammalian and bacterial expression vectors. .. The amplified PCR product was cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen, V79020) and the bacterial expression vector pET21b(+) (Novagen, 69741).

    Article Title:
    Article Snippet: .. After transformation into the yeast reporter strain Saccharomyces cerevisiae CG1945 (Clontech) a human brain cDNA library in the Gal4 activation domain fish vector pACTII (Clontech) was screened for interacting proteins as described. ..

    Article Title: Identification of Splicing Regulatory Activity of ATXN1 and Its Associated Domains
    Article Snippet: .. In the first step, ATXN1 was amplified from the Clontech Human Brain cDNA Library (Clontech, Mountain View, CA, USA) using the primer set nest-ATXN1-Fw and nest-ATXN1-Rv. ..

    Article Title: Variants in MTNAP1 underlie a neurodegenerative disorder by impairing mitochondrial stability.
    Article Snippet: .. AR TI CL E IN P RE SS Oligonucleotide Sequence Forward primer 1 (P1 and P2) 5’-ATGAGTGATAATCCACCCAG-3’ Reverse primer 1 (P1 and P2) 5’-GCTGGCTTGGACTGATAAAC-3’ Forward primer 2 (P3) 5’-GGGAGCTGTACAAAAAGG-3’ Reverse primer 2 (P3) 5’-GAAACTCCAGCTACAACA-3’ Gene cloning and mutagenesis of MTNAP1 Full-length human MTNAP1 (NM_017941.6) cDNA was amplified from a human brain cDNA library (Takara Bio, 637242) using gene-specific primers (Table 2) incorporating restriction enzyme sites compatible with mammalian and bacterial expression vectors. .. The amplified PCR product was cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen, V79020) and the bacterial expression vector pET21b(+) (Novagen, 69741).

    Article Title: Identification of Splicing Regulatory Activity of ATXN1 and Its Associated Domains.
    Article Snippet: .. In the first step, ATXN1 was amplified from the Clontech Human Brain cDNA Library (Clontech, Mountain View, CA, USA) using the primer set nest-ATXN1-Fw and nest-ATXN1-Rv. ..

    Article Title: Variants in LRRC7 lead to intellectual disability, autism, aggression and abnormal eating behaviors.
    Article Snippet: .. Yeast two hybrid screening was performed against a human brain cDNA library in pACT2 using protocols obtained from the manufacturer of the library (Clontech), with the reconstitution of the His3 gene as well as β-Galactosidase staining as selectable markers in the CG1945 strain57. ..

    Expressing:

    Article Title: Variants in MTNAP1 underlie a neurodegenerative disorder by impairing mitochondrial stability
    Article Snippet: .. Full-length human MTNAP1 ( NM_017941.6 ) cDNA was amplified from a human brain cDNA library (Takara Bio, 637242) using gene-specific primers (Table ) incorporating restriction enzyme sites compatible with mammalian and bacterial expression vectors. .. The amplified PCR product was cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen, V79020) and the bacterial expression vector pET21b(+) (Novagen, 69741).

    Article Title: Variants in MTNAP1 underlie a neurodegenerative disorder by impairing mitochondrial stability.
    Article Snippet: .. AR TI CL E IN P RE SS Oligonucleotide Sequence Forward primer 1 (P1 and P2) 5’-ATGAGTGATAATCCACCCAG-3’ Reverse primer 1 (P1 and P2) 5’-GCTGGCTTGGACTGATAAAC-3’ Forward primer 2 (P3) 5’-GGGAGCTGTACAAAAAGG-3’ Reverse primer 2 (P3) 5’-GAAACTCCAGCTACAACA-3’ Gene cloning and mutagenesis of MTNAP1 Full-length human MTNAP1 (NM_017941.6) cDNA was amplified from a human brain cDNA library (Takara Bio, 637242) using gene-specific primers (Table 2) incorporating restriction enzyme sites compatible with mammalian and bacterial expression vectors. .. The amplified PCR product was cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen, V79020) and the bacterial expression vector pET21b(+) (Novagen, 69741).

    Transformation Assay:

    Article Title:
    Article Snippet: .. After transformation into the yeast reporter strain Saccharomyces cerevisiae CG1945 (Clontech) a human brain cDNA library in the Gal4 activation domain fish vector pACTII (Clontech) was screened for interacting proteins as described. ..

    Activation Assay:

    Article Title:
    Article Snippet: .. After transformation into the yeast reporter strain Saccharomyces cerevisiae CG1945 (Clontech) a human brain cDNA library in the Gal4 activation domain fish vector pACTII (Clontech) was screened for interacting proteins as described. ..

    Plasmid Preparation:

    Article Title:
    Article Snippet: .. After transformation into the yeast reporter strain Saccharomyces cerevisiae CG1945 (Clontech) a human brain cDNA library in the Gal4 activation domain fish vector pACTII (Clontech) was screened for interacting proteins as described. ..

    Sequencing:

    Article Title: Variants in MTNAP1 underlie a neurodegenerative disorder by impairing mitochondrial stability.
    Article Snippet: .. AR TI CL E IN P RE SS Oligonucleotide Sequence Forward primer 1 (P1 and P2) 5’-ATGAGTGATAATCCACCCAG-3’ Reverse primer 1 (P1 and P2) 5’-GCTGGCTTGGACTGATAAAC-3’ Forward primer 2 (P3) 5’-GGGAGCTGTACAAAAAGG-3’ Reverse primer 2 (P3) 5’-GAAACTCCAGCTACAACA-3’ Gene cloning and mutagenesis of MTNAP1 Full-length human MTNAP1 (NM_017941.6) cDNA was amplified from a human brain cDNA library (Takara Bio, 637242) using gene-specific primers (Table 2) incorporating restriction enzyme sites compatible with mammalian and bacterial expression vectors. .. The amplified PCR product was cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen, V79020) and the bacterial expression vector pET21b(+) (Novagen, 69741).

    Cloning:

    Article Title: Variants in MTNAP1 underlie a neurodegenerative disorder by impairing mitochondrial stability.
    Article Snippet: .. AR TI CL E IN P RE SS Oligonucleotide Sequence Forward primer 1 (P1 and P2) 5’-ATGAGTGATAATCCACCCAG-3’ Reverse primer 1 (P1 and P2) 5’-GCTGGCTTGGACTGATAAAC-3’ Forward primer 2 (P3) 5’-GGGAGCTGTACAAAAAGG-3’ Reverse primer 2 (P3) 5’-GAAACTCCAGCTACAACA-3’ Gene cloning and mutagenesis of MTNAP1 Full-length human MTNAP1 (NM_017941.6) cDNA was amplified from a human brain cDNA library (Takara Bio, 637242) using gene-specific primers (Table 2) incorporating restriction enzyme sites compatible with mammalian and bacterial expression vectors. .. The amplified PCR product was cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen, V79020) and the bacterial expression vector pET21b(+) (Novagen, 69741).

    Mutagenesis:

    Article Title: Variants in MTNAP1 underlie a neurodegenerative disorder by impairing mitochondrial stability.
    Article Snippet: .. AR TI CL E IN P RE SS Oligonucleotide Sequence Forward primer 1 (P1 and P2) 5’-ATGAGTGATAATCCACCCAG-3’ Reverse primer 1 (P1 and P2) 5’-GCTGGCTTGGACTGATAAAC-3’ Forward primer 2 (P3) 5’-GGGAGCTGTACAAAAAGG-3’ Reverse primer 2 (P3) 5’-GAAACTCCAGCTACAACA-3’ Gene cloning and mutagenesis of MTNAP1 Full-length human MTNAP1 (NM_017941.6) cDNA was amplified from a human brain cDNA library (Takara Bio, 637242) using gene-specific primers (Table 2) incorporating restriction enzyme sites compatible with mammalian and bacterial expression vectors. .. The amplified PCR product was cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen, V79020) and the bacterial expression vector pET21b(+) (Novagen, 69741).

    Two Hybrid Screening:

    Article Title: Variants in LRRC7 lead to intellectual disability, autism, aggression and abnormal eating behaviors.
    Article Snippet: .. Yeast two hybrid screening was performed against a human brain cDNA library in pACT2 using protocols obtained from the manufacturer of the library (Clontech), with the reconstitution of the His3 gene as well as β-Galactosidase staining as selectable markers in the CG1945 strain57. ..

    Staining:

    Article Title: Variants in LRRC7 lead to intellectual disability, autism, aggression and abnormal eating behaviors.
    Article Snippet: .. Yeast two hybrid screening was performed against a human brain cDNA library in pACT2 using protocols obtained from the manufacturer of the library (Clontech), with the reconstitution of the His3 gene as well as β-Galactosidase staining as selectable markers in the CG1945 strain57. ..



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    Image Search Results


    Molecular cloning of novel RGS6 splice forms from human brain. ( A ) Splicing diagram of known RGS6 splice forms. Location of primers used for PCR-based amplification of RGS6 transcripts from a human whole brain cDNA library are shown in red. ( B ) Representative plasmid restriction digest (right) or nested PCR (down) indicating positive hits whose size is larger than known RGS6 splice forms. Each gel has a confirmed RGS6Lα1(+GGL) transcript (largest known splice form) indicated as well as yellow asterisks denoting transcripts encoding RGS6LA3α1(+GGL) which encodes RGS6B.

    Journal: bioRxiv

    Article Title: Molecular cloning of a novel, nervous system-specific RGS6 isoform lacking canonical G protein regulatory effects and with dominant negative actions

    doi: 10.64898/2026.05.08.723811

    Figure Lengend Snippet: Molecular cloning of novel RGS6 splice forms from human brain. ( A ) Splicing diagram of known RGS6 splice forms. Location of primers used for PCR-based amplification of RGS6 transcripts from a human whole brain cDNA library are shown in red. ( B ) Representative plasmid restriction digest (right) or nested PCR (down) indicating positive hits whose size is larger than known RGS6 splice forms. Each gel has a confirmed RGS6Lα1(+GGL) transcript (largest known splice form) indicated as well as yellow asterisks denoting transcripts encoding RGS6LA3α1(+GGL) which encodes RGS6B.

    Article Snippet: Full-length cDNAs encoding novel RGS6 splice forms were amplified from a Marathon ready human brain cDNA library (Clontech; Mountain View, CA, USA) using a PCR-based strategy.

    Techniques: Molecular Cloning, Amplification, cDNA Library Assay, Plasmid Preparation, Nested PCR

    RGS6B is an RGS6L(+GGL) isoform containing A3 and the α terminal exon (RGS6LA3α1(+GGL)). ( A ) Schematic diagram of the exon splicing scheme comparing RGS6Lα1(+GGL) (RGS6L) and RGS6LA3α1(+GGL) (RGS6B). mRNA sequence conservation of exon A3 between mouse and human is depicted below with the consensus sequence from 100 vertebrate species extracted by PhyloP. PCR amplification of exon A3 containing mRNA transcripts from select mouse tissues ( B ) or human cDNA libraries ( C ). Plasmids encoding RGS6Lα1, RGS6Lα2 and RGS6LA3α1 (all +GGL) are used as negative and positive controls, respectively. LNG, lung; KDN, kidney; LVR, liver; SPL, spleen; CTX, cortex; MB, midbrain; CRB, cerebellum; HIP, hippocampus; MSC, muscle; HRT, heart; PNC, peripheral nervous system; BR, whole brain. ( D ) HEK293T cells were transfected with a plasmid encoding RGS6LA3α1(+GGL) to confirm co-migration with mouse RGS6B via immunoblotting. ( E ) shRNA or miRNA constructs targeting the A3 exon were introduced into mouse primary cortical astrocytes. The ratio of RGS6B: RGS6L was determined via immunoblot and quantified from 4 independent experiments. α Tubulin serves as a loading control for immunoblots. Data were analyzed by one sample t-test to detect deviation of each group from 100% (scramble RNAi control). *P<0.05, ***P<0.001. Data are expressed as mean ± S.E.M.

    Journal: bioRxiv

    Article Title: Molecular cloning of a novel, nervous system-specific RGS6 isoform lacking canonical G protein regulatory effects and with dominant negative actions

    doi: 10.64898/2026.05.08.723811

    Figure Lengend Snippet: RGS6B is an RGS6L(+GGL) isoform containing A3 and the α terminal exon (RGS6LA3α1(+GGL)). ( A ) Schematic diagram of the exon splicing scheme comparing RGS6Lα1(+GGL) (RGS6L) and RGS6LA3α1(+GGL) (RGS6B). mRNA sequence conservation of exon A3 between mouse and human is depicted below with the consensus sequence from 100 vertebrate species extracted by PhyloP. PCR amplification of exon A3 containing mRNA transcripts from select mouse tissues ( B ) or human cDNA libraries ( C ). Plasmids encoding RGS6Lα1, RGS6Lα2 and RGS6LA3α1 (all +GGL) are used as negative and positive controls, respectively. LNG, lung; KDN, kidney; LVR, liver; SPL, spleen; CTX, cortex; MB, midbrain; CRB, cerebellum; HIP, hippocampus; MSC, muscle; HRT, heart; PNC, peripheral nervous system; BR, whole brain. ( D ) HEK293T cells were transfected with a plasmid encoding RGS6LA3α1(+GGL) to confirm co-migration with mouse RGS6B via immunoblotting. ( E ) shRNA or miRNA constructs targeting the A3 exon were introduced into mouse primary cortical astrocytes. The ratio of RGS6B: RGS6L was determined via immunoblot and quantified from 4 independent experiments. α Tubulin serves as a loading control for immunoblots. Data were analyzed by one sample t-test to detect deviation of each group from 100% (scramble RNAi control). *P<0.05, ***P<0.001. Data are expressed as mean ± S.E.M.

    Article Snippet: Full-length cDNAs encoding novel RGS6 splice forms were amplified from a Marathon ready human brain cDNA library (Clontech; Mountain View, CA, USA) using a PCR-based strategy.

    Techniques: Sequencing, Amplification, Transfection, Plasmid Preparation, Migration, Western Blot, shRNA, Construct, Control